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tsne functions  (Becton Dickinson)
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Becton Dickinson tsne functions
<t>Flow</t> <t>cytometry</t> was performed on PBMC from normal donors, HVL, LVL and LVL post control using the Zombie Yellow live/dead stain and antibodies for CD3, TIM3, CD38, TIGIT, PD1, CTLA4, HLA-DR, LAG3, and CD8. A) Cells identified as live (Zombie Yellow-) T-cells (CD3+) were clustered using the FlowSOM plugin in FlowJo. B) <t>tSNE</t> analysis and plotting was performed on the same live T-cell populations using the associated function in FlowJo. Final plot was colored according to the clusters identified in panel A. Cells from human subjects corresponding to C) HIV negative (ND), D) HVL, E) LVL, F) LVL post control, and G) HVL on ART were mapped back onto the tSNE plot. Circled populations are those unique to either LVLs, LVLs post control, or HVLs on ART and are labelled with an identifying number that corresponds to the cluster (A) of which they are a sub-population.
Tsne Functions, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Flow cytometry was performed on PBMC from normal donors, HVL, LVL and LVL post control using the Zombie Yellow live/dead stain and antibodies for CD3, TIM3, CD38, TIGIT, PD1, CTLA4, HLA-DR, LAG3, and CD8. A) Cells identified as live (Zombie Yellow-) T-cells (CD3+) were clustered using the FlowSOM plugin in FlowJo. B) tSNE analysis and plotting was performed on the same live T-cell populations using the associated function in FlowJo. Final plot was colored according to the clusters identified in panel A. Cells from human subjects corresponding to C) HIV negative (ND), D) HVL, E) LVL, F) LVL post control, and G) HVL on ART were mapped back onto the tSNE plot. Circled populations are those unique to either LVLs, LVLs post control, or HVLs on ART and are labelled with an identifying number that corresponds to the cluster (A) of which they are a sub-population.

Journal: bioRxiv

Article Title: HIV-1 controllers possess a unique CD8+ T-cell activation phenotype and loss of control is associated with increased expression of exhaustion markers

doi: 10.1101/2024.04.09.588737

Figure Lengend Snippet: Flow cytometry was performed on PBMC from normal donors, HVL, LVL and LVL post control using the Zombie Yellow live/dead stain and antibodies for CD3, TIM3, CD38, TIGIT, PD1, CTLA4, HLA-DR, LAG3, and CD8. A) Cells identified as live (Zombie Yellow-) T-cells (CD3+) were clustered using the FlowSOM plugin in FlowJo. B) tSNE analysis and plotting was performed on the same live T-cell populations using the associated function in FlowJo. Final plot was colored according to the clusters identified in panel A. Cells from human subjects corresponding to C) HIV negative (ND), D) HVL, E) LVL, F) LVL post control, and G) HVL on ART were mapped back onto the tSNE plot. Circled populations are those unique to either LVLs, LVLs post control, or HVLs on ART and are labelled with an identifying number that corresponds to the cluster (A) of which they are a sub-population.

Article Snippet: Clustering and t-Distributed Stochastic Neighbor Embedding (tSNE) dimensionality reduction of flow cytometry data was performed using the FlowSOM and tSNE functions available within FlowJo v10.

Techniques: Flow Cytometry, Staining